Wednesday, August 28, 2019
Data analysis task Assignment Example | Topics and Well Written Essays - 500 words
Data analysis task - Assignment Example Both of the genes contribute fragments to the gel that have been sectioned by the enzyme. These portions have complementary bases to the probe, thus forming more bands. Simply, there is a copy of R and r portions in the heterozygote. The lighter bands represent homozygote state. That is, there is less DNA to map. This can only imply that half the DNA is present. 7) When the blot was probed at high stringency with a probe made with DNA that flanks the R gene (as seen in Figure 1) a smear typical of thousands of hybridising bands was seen. How could this be explained? 8) The turnaround time for Southern Blot analysis is typically around 10 days. Now that you have identified the region in which the mutation lies explain with detail how a faster test could be designed? Now that the portion has been identified, a faster test would only need one to cut the DNA at the specific location. After that has been done, the cut portion is denatured, pre hybridized and transferred to a gel block. Alternatively, it could be run through PCR and analysed using an electronic DNA
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